IMMUNOHISTOCHEMICAL TARGETING OF HLA-G ANTIGEN IN MISCARRIAGE PLACENTAL TISSUES OF IRAQI WOMEN INFECTED WITH HUMAN HERPES VIRUSES TYPES 1, 2, 4 AND 5
Background: Microbial infections represent a major cause of miscarriage, of which viruses appear to be the most frequently involved pathogens. Among many viral causes of miscarriage, maternal infections caused by Herpes Virus type 1and 2, Cytomegalovirus (CMV) and Epstein-Barr virus infections are important causes. Among many possible causes, HLA-G was implicated in immune mechanisms, and might be involved, in pregnancy loss. Methods: Immunohistochemistry technique was used to detect placental infection with Herpes Virus type 1 and 2, Cytomegalovirus, and Epstein-Barr virus as well as HLAG in 40 women with spontaneous miscarriage and in 40 healthy pregnant women in Baghdad/Iraq. Results: The HSV-1 protein was detected in (10 %) of placental tissues from miscarriage women, HSV-2 in (37.5%) , EBV- VCA in (22.5%) , and CMV in (37.5%). The positive HLA-G markers were detected in (55.0%) of placental tissues from women with live-born children and (22.5%) of miscarriage placental tissues group. The association between HLAG with HSV-1 among the placental tissues from miscarriage patients was constituting (5%), (10%) with HSV-2, (7.5%) with EBV and (2.5%) with CMV. Conclusion: In conclusion, this research work could point for an important role and participation of this marker in protection the fetuses during succeeded full-term pregnancies as well as the highest expressions of HLAG with HSV-2, lower rates of each viral agents especially in the placental tissues of healthy pregnant women than other pointing for its important protecting role. Key Words: Herpes Virus type 1 and 2, Cytomegalovirus; Epstein-Barr virus; Immunohistochemistry; Miscarriage; Placenta; HLAG.